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bsaji  (New England Biolabs)


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    Structured Review

    New England Biolabs bsaji
    Partial DNA sequence of domain II of the Ae. <t>albopictus</t> <t>VGSC</t> gene. Red arrows indicate the forward and reverse primer-corresponding region. The <t>BsaJI</t> restriction site, and partial region of exon/intron are presented
    Bsaji, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsaji/BsaJI/pmc12642244-82-34-38
    Average 93 stars, based on 138 article reviews
    bsaji - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Development and application of a PCR–RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China"

    Article Title: Development and application of a PCR–RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China

    Journal: Parasites & Vectors

    doi: 10.1186/s13071-025-07116-z

    Partial DNA sequence of domain II of the Ae. albopictus VGSC gene. Red arrows indicate the forward and reverse primer-corresponding region. The BsaJI restriction site, and partial region of exon/intron are presented
    Figure Legend Snippet: Partial DNA sequence of domain II of the Ae. albopictus VGSC gene. Red arrows indicate the forward and reverse primer-corresponding region. The BsaJI restriction site, and partial region of exon/intron are presented

    Techniques Used: Sequencing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Development and application of a PCR-RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China.
    Article Snippet: .. The restriction digestion reaction was performed in a 20 μl volume, consisting of 2 μl of 10× rCutSmartTM Buffer (B6004, New England Biolabs), 5 μl of the VGSC 1016 PCR amplicon, 1 μl of BsaJI (10 U/μl) (R0536, New England Biolabs), and 12 μl of ddH2O. ..

    Article Title: GLIS3 rs7034200 and ADRB3 rs4994 genetic variants associated with an increased risk of gestational diabetes mellitus in Chinese women: a case-control study
    Article Snippet: .. After PCR amplification, a 3.5 μL PCR products of the rs4994 SNP (210 bp) or 5 μL rs7034200 SNP (387 bp) was digested using 3.5 U of PflMI and 2 U of BsaJI (New England Biolabs, Inc.), respectively. ..

    Article Title: Development and application of a PCR–RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China
    Article Snippet: .. The restriction digestion reaction was performed in a 20 μl volume, consisting of 2 μl of 10× rCutSmartTM Buffer (B6004, New England Biolabs), 5 μl of the VGSC 1016 PCR amplicon, 1 μl of BsaJI (10 U/μl) (R0536, New England Biolabs), and 12 μl of ddH 2 O. ..

    Article Title: GLIS3 rs7034200 and ADRB3 rs4994 genetic variants associated with an increased risk of gestational diabetes mellitus in Chinese women: a case-control study.
    Article Snippet: .. After PCR amplification, a 3.5 μL PCR products of the rs4994 SNP (210 bp) or 5 μL rs7034200 SNP (387 bp) was digested using 3.5 U of PflMI and 2 U of BsaJI (New England Biolabs, Inc.), respectively. ..

    Article Title: Genetic Variants of the Receptor Activator Nuclear of κB Ligand Gene Increase the Risk of Rheumatoid Arthritis in a Mexican Mestizo Population: A Case–Control Study
    Article Snippet: .. The 552 bp PCR product was incubated at 37 °C with 10U of BsaJI (New England BioLabs, Ipswich, MA, USA) restriction endonuclease, which cuts the PCR product into two fragments of 356 and 196 bp in the presence of the C (Silvestre allele). ..

    Article Title: Predicting colorectal cancer risk in FAP patients using patient-specific organoids.
    Article Snippet: .. FAP1 PCR products were cleaved with NurI restriction enzyme (R3192S, New England Biolabs, Ipswich, MA, USA) and FAP2 products were cleaved with BsaJI (R0536S, New England Biolabs, Ipswich, MA, USA) restriction enzyme which diagnosed the WT allele. .. For determining mutation correction following CRISPR, DNA was extracted from FAP-hESCs using a Quick genomic DNA MiniPrep Kit (ZRD3025, ZYMO, Orange, CA, USA) according to the manufacturer’s instructions.

    Article Title: Predicting colorectal cancer risk in FAP patients using patient-specific organoids
    Article Snippet: For determining mutation correction following CRISPR, DNA was extracted from FAP-hESCs using a Quick genomic DNA MiniPrep Kit (ZRD3025, ZYMO, Orange, CA, USA) according to the manufacturer’s instructions. .. FAP1 PCR products were cleaved with NurI restriction enzyme (R3192S, New England Biolabs, Ipswich, MA, USA) and FAP2 products were cleaved with BsaJI (R0536S, New England Biolabs, Ipswich, MA, USA) restriction enzyme which diagnosed the WT allele. ..

    Amplification:

    Article Title: Development and application of a PCR-RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China.
    Article Snippet: .. The restriction digestion reaction was performed in a 20 μl volume, consisting of 2 μl of 10× rCutSmartTM Buffer (B6004, New England Biolabs), 5 μl of the VGSC 1016 PCR amplicon, 1 μl of BsaJI (10 U/μl) (R0536, New England Biolabs), and 12 μl of ddH2O. ..

    Article Title: GLIS3 rs7034200 and ADRB3 rs4994 genetic variants associated with an increased risk of gestational diabetes mellitus in Chinese women: a case-control study
    Article Snippet: .. After PCR amplification, a 3.5 μL PCR products of the rs4994 SNP (210 bp) or 5 μL rs7034200 SNP (387 bp) was digested using 3.5 U of PflMI and 2 U of BsaJI (New England Biolabs, Inc.), respectively. ..

    Article Title: Development and application of a PCR–RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China
    Article Snippet: .. The restriction digestion reaction was performed in a 20 μl volume, consisting of 2 μl of 10× rCutSmartTM Buffer (B6004, New England Biolabs), 5 μl of the VGSC 1016 PCR amplicon, 1 μl of BsaJI (10 U/μl) (R0536, New England Biolabs), and 12 μl of ddH 2 O. ..

    Article Title: GLIS3 rs7034200 and ADRB3 rs4994 genetic variants associated with an increased risk of gestational diabetes mellitus in Chinese women: a case-control study.
    Article Snippet: .. After PCR amplification, a 3.5 μL PCR products of the rs4994 SNP (210 bp) or 5 μL rs7034200 SNP (387 bp) was digested using 3.5 U of PflMI and 2 U of BsaJI (New England Biolabs, Inc.), respectively. ..

    Incubation:

    Article Title: Genetic Variants of the Receptor Activator Nuclear of κB Ligand Gene Increase the Risk of Rheumatoid Arthritis in a Mexican Mestizo Population: A Case–Control Study
    Article Snippet: .. The 552 bp PCR product was incubated at 37 °C with 10U of BsaJI (New England BioLabs, Ipswich, MA, USA) restriction endonuclease, which cuts the PCR product into two fragments of 356 and 196 bp in the presence of the C (Silvestre allele). ..



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    Partial DNA sequence of domain II of the Ae. <t>albopictus</t> <t>VGSC</t> gene. Red arrows indicate the forward and reverse primer-corresponding region. The <t>BsaJI</t> restriction site, and partial region of exon/intron are presented
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    Image Search Results


    Partial DNA sequence of domain II of the Ae. albopictus VGSC gene. Red arrows indicate the forward and reverse primer-corresponding region. The BsaJI restriction site, and partial region of exon/intron are presented

    Journal: Parasites & Vectors

    Article Title: Development and application of a PCR–RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China

    doi: 10.1186/s13071-025-07116-z

    Figure Lengend Snippet: Partial DNA sequence of domain II of the Ae. albopictus VGSC gene. Red arrows indicate the forward and reverse primer-corresponding region. The BsaJI restriction site, and partial region of exon/intron are presented

    Article Snippet: The restriction digestion reaction was performed in a 20 μl volume, consisting of 2 μl of 10× rCutSmartTM Buffer (B6004, New England Biolabs), 5 μl of the VGSC 1016 PCR amplicon, 1 μl of BsaJI (10 U/μl) (R0536, New England Biolabs), and 12 μl of ddH 2 O.

    Techniques: Sequencing